Application of RNAi technology in forest trees.
Publication: RNAi for plant improvement and protection
pp. 54-71
Abstract
A diverse set of small RNAs is involved in the regulation of genome organization and gene expression in plants. These regulatory sRNAs play a central role for RNA in evolution and ontogeny in complex organisms, including forest tree species, providers of indispensable ecosystem services. RNA interference is a process that inhibits gene expression by double-stranded RNA and thus causes the degradation of target messenger RNA molecules. Targeted gene silencing by RNAi has been utilized in various crop plants in order to enhance their characteristics. For forest tree species, most of the successful RNAi modification has been conducted in poplar. Over the past 20 years, successful RNAi-mediated suppression of gene expression has been achieved with a variety of economically important traits. Moreover, the stability of RNAi-mediated transgene suppression has been confirmed in field-grown poplars. In this chapter, we describe examples of successful RNAi applications mainly in poplar but also provide some information about application of RNAi in pest control in forest tree species. Advantages and disadvantages of this technology with respect to the particular features of forest tree species will be discussed.
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Pages: 54 - 71
Editors: Bruno Mezzetti [email protected], Department of Agricultural Food and Environmental Sciences Università Politecnica delle Marche Via Brecce Bianche 60100 Ancona Italy , Jeremy Sweet [email protected], Sweet Environmental Consultants 6 Green St Cambridge CB24 5JA UK , and Lorenzo Burgos [email protected], Grupo de Biotecnología de Frutales Departamento de Mejora CEBAS-CSIC Campus Universitario de Espinardo Edificio no 25 30100 Murcia Spain
ISBN (ePDF): 978-1-78924-890-6
ISBN (ePub): 978-1-78924-891-3
ISBN (Hardback): 978-1-78924-889-0
Copyright
Open AccessThis article is distributed under the terms of the Attribution-NonCommercial-NoDerivatives 4.0 International (https://creativecommons.org/licenses/by-nc-nd/4.0/).
History
Cover date: 15 March 2021
Published online: 6 April 2021
Language
English
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